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Comparison of two combinations of cyanine dyes for prelabelling and gel electrophoresis
Author(s) -
Tsolakos Nikos,
Techanukul Tanasit,
Wallington Amanda,
Zhao Yuan,
Jones Christopher,
Nagy Judit,
Wheeler Jun X.
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800563
Subject(s) - cyanine , chromatography , chemistry , proteome , gel electrophoresis , sample preparation , labelling , fluorescence , biochemistry , physics , quantum mechanics
We report the use of IC‐OSu ethyl‐Cy3 and ethyl‐Cy5 N ‐hydroxysuccinimide ester (NHS) cyanine dyes, which have similar chemical properties as the CyDye™ DIGE fluor minimal dyes for pre‐electrophoresis labelling. Multiple sample analyses in different laboratories indicate that the use of IC‐OSu ethyl‐Cy3 and ethyl‐Cy5 NHS ester cyanine dyes produces equivalent results to those obtained with DIGE CyDyes, and allows sample multiplexing and accurate quantitation for differential proteome analysis.