Unraveling the S ‐nitrosoproteome: Tools and strategies

One of the major tasks to be accomplished in the postgenomic era is the characterization of PTMs in proteins. The S ‐nitrosation of protein thiols is a redox‐based PTM that modulating enzymatic activity, subcellular localization, complex formation, and degradation of proteins, largely contributes to the complexity of cellular proteomes. Although the detection of S ‐nitrosated proteins is problematical due to the lability of S ‐nitrosothiols, with the improvement of molecular tools an increasing range of proteins has been shown to undergo S ‐nitrosation. We here review recent proteomic approaches for the systematic assessment of potential targets for protein S ‐nitrosation. The development of new analytical methods and strategies over the past several years now allows us to investigate the nitrosoproteome on a global scale.