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POSTMan (POST‐translational modification analysis), a software application for PTM discovery
Author(s) -
Arntzen Magnus Ø.,
Osland Christoffer Leif,
Raa Christopher RaschOlsen,
Kopperud Reidun,
Døskeland SteinOve,
Lewis Aurélia E.,
D'Santos Clive S.
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800500
Subject(s) - posttranslational modification , computational biology , software , computer science , biology , operating system , biochemistry , enzyme
Post‐translationally modified peptides present in low concentrations are often not selected for CID, resulting in no sequence information for these peptides. We have developed a software POSTMan (POST‐translational Modification analysis) allowing post‐translationally modified peptides to be targeted for fragmentation. The software aligns LC‐MS runs (MS 1 data) between individual runs or within a single run and isolates pairs of peptides which differ by a user defined mass difference (post‐translationally modified peptides). The method was validated for acetylated peptides and allowed an assessment of even the basal protein phosphorylation of phenylalanine hydroxylase (PHA) in intact cells.