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Using the protein chip interface with quadrupole time‐of‐flight mass spectrometry to directly identify peaks in SELDI profiles – initial evaluation using low molecular weight serum peaks
Author(s) -
Peng Jianhe,
Stanley Anthea J.,
Cairns David,
Selby Peter J.,
Banks Rosamonde E.
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800424
Subject(s) - quadrupole time of flight , mass spectrometry , tandem mass tag , computational biology , tandem mass spectrometry , chromatography , protein chip , isobaric labeling , proteomics , chemistry , identification (biology) , acetylation , analytical chemistry (journal) , bioinformatics , protein mass spectrometry , biology , quantitative proteomics , biochemistry , gene , botany
Mass spectrometric profiling, particularly in the form of SELDI, has been used in many studies, particularly in attempts to generate diagnostic serum profiles. Several studies have generated promising results but one of the limitations is the inability to identify easily potential discriminatory peaks. This may enable specific assays to be developed and increased biological insight. We describe the first systematic technical evaluation of the ProteinChip interface coupled to a tandem mass spectrometer which allows direct sequencing of peptides <6000 Da, and describe the direct sequence identification of 21 peaks commonly observed in serum samples. Additionally we describe for the first time the use of on‐chip acetylation to assist in the validation of sequence identification.