Two‐dimensional proteome reference maps for the human pathogenic filamentous fungus   Aspergillus fumigatus | Zendy

Vödisch Martin | Zendy; Albrecht Daniela | Zendy; Leßing Franziska | Zendy; Schmidt André D. | Zendy; Winkler Robert | Zendy; Guthke Reinhard | Zendy; Brakhage Axel A. | Zendy; Kniemeyer Olaf | Zendy

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Two‐dimensional proteome reference maps for the human pathogenic filamentous fungus Aspergillus fumigatus

Author(s) -

Vödisch Martin,

Albrecht Daniela,

Leßing Franziska,

Schmidt André D.,

Winkler Robert,

Guthke Reinhard,

Brakhage Axel A.,

Kniemeyer Olaf

Publication year - 2009

Publication title -

proteomics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.26

H-Index - 167

eISSN - 1615-9861

pISSN - 1615-9853

DOI - 10.1002/pmic.200800394

Subject(s) - aspergillus fumigatus , proteome , biology , fungus , microbiology and biotechnology , pathogenic fungus , fungal protein , proteomics , pathogen , fungal pathogen , filamentous fungus , biochemistry , enzyme , peptide sequence , botany , gene

The filamentous fungus Aspergillus fumigatus has become the most important airborne fungal pathogen causing life‐threatening infections in immunosuppressed patients. We established a 2‐D reference map for A. fumigatus . Using MALDI‐TOF‐MS/MS, we identified 381 spots representing 334 proteins. Proteins involved in cellular metabolism, protein synthesis, transport processes and cell cycle were most abundant. Furthermore, we established a protocol for the isolation of mitochondria of A. fumigatus and developed a mitochondrial proteome reference map. 147 proteins represented by 234 spots were identified.

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