Premium
Two‐dimensional proteome reference maps for the human pathogenic filamentous fungus Aspergillus fumigatus
Author(s) -
Vödisch Martin,
Albrecht Daniela,
Leßing Franziska,
Schmidt André D.,
Winkler Robert,
Guthke Reinhard,
Brakhage Axel A.,
Kniemeyer Olaf
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800394
Subject(s) - aspergillus fumigatus , proteome , biology , fungus , microbiology and biotechnology , pathogenic fungus , fungal protein , proteomics , pathogen , fungal pathogen , filamentous fungus , biochemistry , enzyme , peptide sequence , botany , gene
The filamentous fungus Aspergillus fumigatus has become the most important airborne fungal pathogen causing life‐threatening infections in immunosuppressed patients. We established a 2‐D reference map for A. fumigatus . Using MALDI‐TOF‐MS/MS, we identified 381 spots representing 334 proteins. Proteins involved in cellular metabolism, protein synthesis, transport processes and cell cycle were most abundant. Furthermore, we established a protocol for the isolation of mitochondria of A. fumigatus and developed a mitochondrial proteome reference map. 147 proteins represented by 234 spots were identified.