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Protein Standard Absolute Quantification (PSAQ) for improved investigation of staphylococcal food poisoning outbreaks
Author(s) -
Dupuis Alain,
Hennekinne JacquesAntoine,
Garin Jérôme,
Brun Virginie
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800326
Subject(s) - outbreak , food poisoning , enterotoxin , food contaminant , food microbiology , environmental health , microbiology and biotechnology , biology , medicine , food science , virology , bacteria , biochemistry , genetics , escherichia coli , gene
Staphylococcal enterotoxins are major causing agents of food‐borne diseases. Their detection in food remnants for risk assessment or food poisoning outbreaks investigation suffers from a lack in comprehensive immunological tools. In this study, we demonstrate that the combination of immunocapture and Protein Standard Absolute Quantification (PSAQ) strategy, which uses isotope‐labeled enterotoxins as internal standards for MS‐based analysis, is powerful to specifically identify and quantify these contaminating agents in food matrices. This approach is believed to significantly improve the elucidation of staphylococcal food poisoning outbreaks.