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Subproteomic tools to increase genome annotation complexity
Author(s) -
Wei Candong,
Peng Junping,
Xiong Zhaohui,
Yang Jian,
Wang Jing,
Jin Qi
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800226
Subject(s) - annotation , computational biology , genome project , genome , biology , computer science , genetics , gene
Comprehensive and precise annotations of short protein‐coding genes are always a challenging task. Here we propose a new design to facilitate the characterization of previously overlooked short protein‐coding genes by integrating a shotgun proteomics method and oligonucleotide array analysis. Using Shigella flexneri as a model, we validate 163 annotated ORFs and 51 hypothetical or putative transcripts at the protein level, and discover four novel short ORFs. This strategy will contribute significantly to comprehensive and accurate genome‐wide annotation, and to our understanding of prokaryotic genome structure.