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A novel solid phase‐ and chemical crosslinking‐based technology for determining protein localization in biological supramolecules
Author(s) -
Horng WeiCheng,
Yen YuanHun,
Chang YenChung
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800216
Subject(s) - proteomics , function (biology) , nanotechnology , postsynaptic potential , materials science , chemistry , biophysics , biological system , biology , biochemistry , microbiology and biotechnology , receptor , gene
A solid phase‐ and chemical crosslinking‐based technology was developed for determining the depths at which various protein constituents reside in a supramolecule. The usefulness of this technology was verified by trials using a synthetic three‐protein complex on glass coverslips. This technology was further applied to investigate the localization of seven major protein components in the postsynaptic density, a landmark supramolecule of the excitatory synapses in mammalian brains. The technology reported here will supplement the already powerful proteomic methodologies in studying the structure/function relationships of supramolecules.