A novel solid phase‐ and chemical crosslinking‐based technology for determining protein localization in biological supramolecules | Zendy

Horng WeiCheng | Zendy; Yen YuanHun | Zendy; Chang YenChung | Zendy

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A novel solid phase‐ and chemical crosslinking‐based technology for determining protein localization in biological supramolecules

Author(s) -

Horng WeiCheng,

Yen YuanHun,

Chang YenChung

Publication year - 2008

Publication title -

proteomics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.26

H-Index - 167

eISSN - 1615-9861

pISSN - 1615-9853

DOI - 10.1002/pmic.200800216

Subject(s) - proteomics , function (biology) , nanotechnology , postsynaptic potential , materials science , chemistry , biophysics , biological system , biology , biochemistry , microbiology and biotechnology , receptor , gene

A solid phase‐ and chemical crosslinking‐based technology was developed for determining the depths at which various protein constituents reside in a supramolecule. The usefulness of this technology was verified by trials using a synthetic three‐protein complex on glass coverslips. This technology was further applied to investigate the localization of seven major protein components in the postsynaptic density, a landmark supramolecule of the excitatory synapses in mammalian brains. The technology reported here will supplement the already powerful proteomic methodologies in studying the structure/function relationships of supramolecules.

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