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Combination of immunohistochemistry and laser ablation ICP mass spectrometry for imaging of cancer biomarkers
Author(s) -
Seuma Jaume,
Bunch Josephine,
Cox Alan,
McLeod Cameron,
Bell Jane,
Murray Cliff
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800167
Subject(s) - laser ablation , tissue microarray , breast cancer , cancer biomarkers , cancer , ablation , immunohistochemistry , pathology , mass spectrometry , biomedical engineering , laser , proteomics , maldi imaging , chemistry , materials science , medicine , chromatography , optics , biochemistry , matrix assisted laser desorption/ionization , physics , gene , organic chemistry , desorption , adsorption
Laser ablation (LA) ICP‐MS has been developed as a new tool for imaging of cancer biomarkers in tissue sections. The distribution of two breast cancer‐associated proteins, MUC‐1 and HER2 was studied based on multiple line rastering of tissue sections and measurement of relevant Au/Ag tagged antibodies bound to the tissue. Comparisons with optical microscopy indicated extremely high sensitivity for the LA technique and sufficiently good resolution to permit fine scale feature mapping at the cellular level. Application to the quantitative assessment of HER2 expression in tissue microarrays was demonstrated.