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Proteomics and phosphoproteomics for the mapping of cellular signalling networks
Author(s) -
Preisinger Christian,
von Kriegsheim Alex,
Matallanas David,
Kolch Walter
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800136
Subject(s) - phosphoproteomics , proteomics , computational biology , systems biology , biology , signalling pathways , proteome , signal transduction , signalling , microbiology and biotechnology , bioinformatics , genetics , phosphorylation , protein phosphorylation , protein kinase a , gene
Proteomics is transitioning from inventory mapping to the mapping of functional cellular contexts. This has been enabled by progress in technologies as well as conceptual strategies. Here, we review recent advances in this area with focus on cellular signalling pathways. We discuss genetics‐based methods such as yeast two hybrid methods as well as biochemistry‐based methods such as two‐dimensional gel electrophoresis, quantitative proteomics, interaction proteomics, and phosphoproteomics. A central tenet is that by its ability to capture dynamic changes in protein expression, localisation and modification modern proteomics has become a powerful tool to map signal transduction pathways and deliver the functional information that will promote insights in cell biology and systems biology.