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Proteomic identification of a PSF/p54nrb heterodimer as RNF43 oncoprotein‐interacting proteins
Author(s) -
Miyamoto Kentaro,
Sakurai Hidetaka,
Sugiura Takeyuki
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800083
Subject(s) - nucleoplasm , immunofluorescence , identification (biology) , immunoprecipitation , microbiology and biotechnology , biology , computational biology , nuclear protein , dna binding protein , chemistry , genetics , transcription factor , gene , nucleus , antibody , botany , nucleolus
RNF43 is an oncogenic RING finger protein overexpressed in colorectal cancer. To dissect its biological functions, we explored RNF43‐interacting proteins by pull‐down assay and MS. We identified a heterodimer, p54nrb and PSF, as RNF43's binding partners and confirmed their physical interaction in vivo by the co‐immunoprecipitation experiment. Immunofluorescence analysis revealed that co‐expression of PSF relocates RNF43 from the nuclear periphery to the nucleoplasm. Thus, proteomic identification of RNF43‐associated proteins sheds light on its dynamic interaction network in nuclear events.