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Comparative proteomic analysis of human mesenchymal and embryonic stem cells: Towards the definition of a mesenchymal stem cell proteomic signature
Author(s) -
Roche Stephane,
Delorme Bruno,
Oostendorp Robert A. J.,
Barbet Romain,
Caton David,
Noel Daniele,
Boumediene Karim,
Papadaki Helen A.,
Cousin Beatrice,
Crozet Carole,
Milhavet Ollivier,
Casteilla Louis,
Hatzfeld Jacques,
Jorgensen Christian,
Charbord Pierre,
Lehmann Sylvain
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800035
Subject(s) - mesenchymal stem cell , stem cell , stem cell transplantation for articular cartilage repair , microbiology and biotechnology , embryonic stem cell , biology , adult stem cell , progenitor cell , amniotic stem cells , adipose tissue , clinical uses of mesenchymal stem cells , endothelial stem cell , immunology , in vitro , biochemistry , gene
Mesenchymal stem cells (MSC) are adult multipotential progenitors which have a high potential in regenerative medicine. They can be isolated from different tissues throughout the body and their homogeneity in terms of phenotype and differentiation capacities is a real concern. To address this issue, we conducted a 2‐DE gel analysis of mesenchymal stem cells isolated from bone marrow (BM), adipose tissue, synovial membrane and umbilical vein wall. We confirmed that BM and adipose tissue derived cells were very similar, which argue for their interchangeable use for cell therapy. We also compared human mesenchymal to embryonic stem cells and showed that umbilical vein wall stem cells, a neo‐natal cell type, were closer to BM cells than to embryonic stem cells. Based on these proteomic data, we could propose a panel of proteins which were the basis for the definition of a mesenchymal stem cell proteomic signature.