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Frequently Made Mistakes in Electrophoresis
Author(s) -
Westermeier Reiner
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200790077
Subject(s) - resolution (logic) , computer science , electrophoresis , high resolution , proteomics , chromatography , chemistry , biology , artificial intelligence , archaeology , geography , genetics , gene
In spite of text books, instrument manuals, product instructions, and web tutorials there are a number of erroneous protocols around, which lead repeatedly to issues during electrophoresis runs and to inadequate results. The relatively low resolution and short running time of miniformat systems often conceals these issues. However, in high‐resolution 2‐D electrophoresis in large format gels, one of the most important separation methods in Proteomics, the consequences of these mistakes become more obvious.