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Precision enhancement of MALDI‐TOF MS using high resolution peak detection and label‐free alignment
Author(s) -
Tracy Maureen B.,
Chen Haijian,
Weaver Dennis M.,
Malyarenko Dariya I.,
Sasinowski Maciek,
Cazares Lisa H.,
Drake Richard R.,
Semmes O. John,
Tracy Eugene R.,
Cooke William E.
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200701146
Subject(s) - replicate , resolution (logic) , reproducibility , spectrometer , spectral line , analytical chemistry (journal) , physics , mathematics , chemistry , computer science , statistics , optics , artificial intelligence , chromatography , astronomy
Abstract We have developed an automated procedure for aligning peaks in multiple TOF spectra that eliminates common timing errors and small variations in spectrometer output. Our method incorporates high‐resolution peak detection, re‐binning, and robust linear data fitting in the time domain. This procedure aligns label‐free (uncalibrated) peaks to minimize the variation in each peak's location from one spectrum to the next, while maintaining a high number of degrees of freedom. We apply our method to replicate pooled‐serum spectra from multiple laboratories and increase peak precision ( t / σ t ) to values limited only by small random errors (with σ t less than one time count in 89 out of 91 instances, 13 peaks in seven datasets). The resulting high precision allowed for an order of magnitude improvement in peak m / z reproducibility. We show that the CV for m / z is 0.01% (100 ppm) for 12 out of the 13 peaks that were observed in all datasets between 2995 and 9297 Da.