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Proteomic analysis of chromium cytotoxicity in cultured rat lung epithelial cells
Author(s) -
Lei Ting,
He QingYu,
Cai Zhen,
Zhou Yuan,
Wang YiLi,
Si LuSheng,
Cai Zongwei,
Chiu JenFu
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200701050
Subject(s) - hexavalent chromium , dna damage , apoptosis , carcinogenesis , toxicity , proteomics , cytotoxicity , carcinogen , blot , microbiology and biotechnology , chemistry , biology , cancer research , chromium , biochemistry , dna , gene , in vitro , organic chemistry
Chromium (Cr) has been widely used in industry for more than one century. Exposure to hexavalent Cr compounds is strongly associated with increasing risk of lung cancer. Extensive researches at DNA level indicated that generation of ROS from the reduction of Cr(VI) leading to DNA damage is the major cause of the toxicity and carcinogenicity of Cr(VI). The present study in cellular and protein levels confirmed that Cr(VI) induced apoptosis of lung epithelial cells (LEC) via ROS generation. To view the differentially expressed proteins in the process of Cr(VI) reduction, subcellular proteomics was applied and allowed the identification of more than 30 proteins with expression alteration. Most of those proteins are correlated with ROS‐elicited responses, which were further validated by Western blotting analysis, induction of p53 pathway and antioxidative treatment. The current findings provided additional evidence in protein level to support the claim that ROS generated during the process of Cr(VI) reduction are involved in the Cr(VI)‐induced toxicity and carcinogenesis.