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A simple and highly successful C‐terminal sequence analysis of proteins by mass spectrometry
Author(s) -
Kuyama Hiroki,
Shima Keisuke,
Sonomura Kazuhiro,
Yamaguchi Minoru,
Ando Eiji,
Nishimura Osamu,
Tsunasawa Susumu
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200701044
Subject(s) - mass spectrometry , chemistry , cyanogen bromide , tandem mass spectrometry , proteomics , computational biology , tandem mass tag , terminal (telecommunication) , sequence (biology) , protein sequencing , posttranslational modification , peptide sequence , combinatorial chemistry , biochemistry , biology , chromatography , quantitative proteomics , computer science , enzyme , gene , telecommunications
A simple and efficient method for C‐terminal sequencing of proteins has long been pursued because it would provide substantial information for identifying the covalent structure, including post‐translational modifications. However, there are still significant impediments to both direct sequencing from C termini of proteins and specific isolation of C‐terminal peptides from proteins. We describe here a highly successful, de novo C‐terminal sequencing method of proteins by employing succinimidyloxycarbonylmethyl tris (2,4,6‐trimethoxyphenyl) phosphonium bromide and mass spectrometry.