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Trypsin‐immobilized fiber core in syringe needle for highly efficient proteolysis
Author(s) -
Wang Sheng,
Chen Zhi,
Yang Pengyuan,
Chen Gang
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200701042
Subject(s) - bioreactor , proteolysis , chromatography , trypsin , fiber , lysozyme , chemistry , digestion (alchemy) , enzyme , biochemistry , organic chemistry
Abstract In this report, a core‐changeable needle enzymatic reactor was developed for highly efficient proteolysis. A piece of enzyme‐immobilized fiber core was inserted into the needle of a syringe pump to form a flow‐through bioreactor. The novel in‐needle bioreactor could be regenerated by changing its fiber core. The feasibility and performance of the unique bioreactor were demonstrated by the tryptic digestion of BSA and lysozyme and the digestion time was significantly reduced to less than 5 s. The digests were identified by MALDI‐TOF MS with sequence coverages comparable to those obtained by the conventional in‐solution tryptic digestion. The present in‐needle bioreactor provides a promising platform for the high‐throughput protein identification.