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Proteomic analysis of zygote and ookinete stages of the avian malaria parasite Plasmodium gallinaceum delineates the homologous proteomes of the lethal human malaria parasite Plasmodium falciparum
Author(s) -
Patra Kailash P.,
Johnson Jeff R.,
Cantin Greg T.,
Yates John R.,
Vinetz Joseph M.
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700727
Subject(s) - plasmodium gallinaceum , biology , zygote , proteome , plasmodium (life cycle) , plasmodium falciparum , parasite hosting , midgut , avian malaria , orfs , virology , gametocyte , malaria , microbiology and biotechnology , genetics , peptide sequence , open reading frame , gene , immunology , embryo , embryogenesis , botany , world wide web , computer science , larva
Delineation of the complement of proteins comprising the zygote and ookinete, the early developmental stages of Plasmodium within the mosquito midgut, is fundamental to understand initial molecular parasite‐vector interactions. The published proteome of Plasmodium falciparum does not include analysis of the zygote/ookinete stages, nor does that of P. berghei include the zygote stage or secreted proteins. P. gallinaceum zygote, ookinete, and ookinete‐secreted/released protein samples were prepared and subjected to Multidimensional protein identification technology (MudPIT). Peptides of P. gallinaceum zygote, ookinete, and ookinete‐secreted proteins were identified by MS/MS, mapped to ORFs (>50 amino acids) in the extent P. gallinaceum whole genome sequence, and then matched to homologous ORFs in P. falciparum. A total of 966 P. falciparum ORFs encoding orthologous proteins were identified; just over 40% of these predicted proteins were found to be hypothetical. A majority of putative proteins with predicted secretory signal peptides or transmembrane domains were hypothetical proteins. This analysis provides a more comprehensive view of the hitherto unknown proteome of the early mosquito midgut stages of P. falciparum . The results underpin more robust study of Plasmodium –mosquito midgut interactions, fundamental to the development of novel strategies of blocking malaria transmission.