Premium
The effect of low pH on protein expression by the probiotic bacterium Lactobacillus reuteri
Author(s) -
Lee KiBeom,
Lee HongGu,
Pi KyungBae,
Choi YunJaie
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700663
Subject(s) - lactobacillus reuteri , probiotic , bacteria , lactic acid , biochemistry , metabolism , amino acid , chemistry , protein biosynthesis , gastrointestinal tract , biology , genetics
Abstract The ability of a lactic acid bacterium to survive passage through the gastrointestinal tract is a key point in its function as a probiotic. In this study, protein synthesis by the probiotic bacterium, Lactobacillus reuteri , was analyzed under transiently decreased pH conditions. L. reuteri cells grown to the midexponential growth phase at 37°C were exposed to transient (1 h) low‐pH stresses from pH 6.8 to pH 5.0, 4.5, or 4.0. 2‐DE allowed us to identify 40 common proteins that were consistently and significantly altered under all three low‐pH conditions. PMF was used to identify these 40 proteins, and functional annotation allowed them to be distributed to six major classes: (i) transport and binding proteins; (ii) transcription–translation; (iii) nucleotide metabolism and amino acid biosynthesis; (iv) carbon energy metabolism; (v) pH homeostasis and stress; and (vi) unassigned. These findings provide new insight into the inducible mechanisms underlying the capacity of gastrointestinal L. reuteri to tolerate acid stress.