Premium
Why 3‐D? Gel‐based microarrays in proteomics
Author(s) -
Rubina Alla Yu.,
Kolchinsky Alexander,
Makarov Alexander A.,
Zasedatelev Alexander S.
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700629
Subject(s) - proteomics , dna microarray , computational biology , biology , genetics , gene , gene expression
Gel‐based microarrays (biochips) consisting of nanoliter and sub‐nanoliter gel drops on hydrophobic substrate are a versatile technology platform for immobilization of proteins and other biopolymers. Biochips provide a highly hydrophilic environment, which stabilizes immobilized molecules and facilitates their interactions with analytes. The probes are immobilized simultaneously with gel polymerization, evenly distributed throughout individual elements, and are easily accessible because of large pores. Each element is an isolated nanotube. Applications of biochips in the studies of protein interactions with other proteins, nucleic acids, and glycans are described. In particular, biochips are compatible with MALDI‐MS. Biochip‐based assay of prostate‐specific antigen became the first protein microarray approved for clinical use by a national regulatory agency. In this review, 3‐D immobilization is compared with mainstream technologies based on surface immobilization.