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Proteomic analyses of Fusarium graminearum grown under mycotoxin‐inducing conditions
Author(s) -
Taylor Rebecca D.,
Saparno Audrey,
Blackwell Barbara,
Anoop Valar,
Gleddie Steve,
Tinker Nicholas A.,
Harris Linda J.
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700610
Subject(s) - mycotoxin , fusarium , biology , proteomics , gene expression , gene , downregulation and upregulation , quantitative proteomics , microbiology and biotechnology , biochemistry , genetics , botany
Non‐gel‐based quantitative proteomics technology was used to profile protein expression differences when Fusarium graminearum was induced to produce trichothecenes in vitro . As F. graminearum synthesizes and secretes trichothecenes early in the cereal host invasion process, we hypothesized that proteins contributing to infection would also be induced under conditions favouring mycotoxin synthesis. Protein samples were extracted from three biological replicates of a time course study and subjected to iTRAQ (isobaric tags for relative and absolute quantification) analysis. Statistical analysis of a filtered dataset of 435 proteins revealed 130 F. graminearum proteins that exhibited significant changes in expression, of which 72 were upregulated relative to their level at the initial phase of the time course. There was good agreement between upregulated proteins identified by 2‐D PAGE/MS/MS and iTRAQ. RT‐PCR and northern hybridization confirmed that genes encoding proteins which were upregulated based on iTRAQ were also transcriptionally active under mycotoxin producing conditions. Numerous candidate pathogenicity proteins were identified using this technique. These will provide leads in the search for mechanisms and markers of host invasion and novel antifungal targets.