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Overexpression of annexin a1 induced by terephthalic acid calculi in rat bladder cancer
Author(s) -
Cui Lunbiao,
Wang Yubang,
Shi Yuan,
Zhang Zhengdong,
Xia Yankai,
Sun Hong,
Wang Shouling,
Chen Jianfeng,
Zhang Wei,
Lu Qiang,
Song Ling,
Wei Qingyi,
Zhang Ruiwen,
Wang Xinru
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700582
Subject(s) - urothelium , bladder cancer , carcinogenesis , immunohistochemistry , pathology , annexin a2 , urinary bladder , biology , transitional cell carcinoma , cancer research , microbiology and biotechnology , staining , cancer , medicine , annexin , genetics
Prolonged cell proliferation in response to irritation by bladder calculi can evoke malignant transformation of the urothelium. However, the molecular mechanisms responsible for calculi‐associated bladder carcinogenesis are unknown. We compared the protein expression pattern of rat bladder transitional cell carcinomas (TCCs) induced by terephthalic acid with that of normal bladder tissues using 2‐DE. Comparative analysis of the respective spot patterns on 2‐DE showed 146 spots that were markedly changed in TCC samples. Subsequently, 56 of the variant protein spots were identified by MALDI‐TOF MS. Among them, overexpression of annexin a1 (ANNA1) in rat TCCs was confirmed by Western blotting and real‐time RT‐PCR analysis. Immunohistochemical staining revealed that ANNA1, usually a cytoplasmic protein in normal urothelium, was translocated to the nucleus in rat bladder cancer cells. In contrast to the animal studies, examination of human clinical specimens showed that ANNA1 expression was reduced in TCC compared to normal urothelium. The expression of ANNA1 was inversely related to the level of differentiation of TCC. Our data suggest that overexpression of ANNA1 is involved in bladder carcinogenesis induced by bladder calculi and that translocation of the protein may be partly responsible for the effect. ANNA1 may serve as a new marker of differentiation for the histopathological grading of human TCC.