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The proteome of gastric lymph in normal and nematode infected sheep
Author(s) -
Goldfinch Gillian M.,
Smith W. David,
Imrie Lisa,
McLean Kevin,
Inglis Neil F.,
Pemberton Alan D.
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700531
Subject(s) - lymph , proteome , lymphatic system , biology , lymph node , pathology , proteomics , medicine , immunology , bioinformatics , biochemistry , gene
Abstract Lymph node cannulation allows the collection of lymph draining from a defined anatomical region. Proteomic analysis of that lymph offers a potentially valuable insight into the immunoinflammatory response of that particular region. In this study, ovine gastric lymph has been used to monitor the proteomic changes occurring in the tissue fluid of the abomasum, in response to infection with the parasitic nematode, Teladorsagia circumcincta . Lymph, collected temporally over an experimental infection period, was analysed by means of 2‐DE and subsequent gel analysis using densitometry software. In addition, the composition of the lymphatic proteome was further explored by means of MALDI‐TOF and MS/MS analyses. The concentration of gelsolin, α‐1 β glycoprotein and haemopexin were altered significantly ( p <0.05) with infection.