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Physiology of Streptococcus thermophilus during the late stage of milk fermentation with special regard to sulfur amino‐acid metabolism
Author(s) -
HerveJimenez Luciana,
Guillouard Isabelle,
Guedon Eric,
Gautier Céline,
Boudebbouze Samira,
Hols Pascal,
Monnet Véronique,
Rul Françoise,
Maguin Emmanuelle
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700489
Subject(s) - streptococcus thermophilus , proteome , biochemistry , lactobacillus , lactic acid , biology , fermentation , metabolism , fermentation starter , bacteria , thermophile , streptococcus bovis , food science , enzyme , genetics , rumen
Streptococcus thermophilus is a thermophilic lactic acid bacterium widely used as starter in the manufacture of dairy products in particular in yoghurt manufacture in combination with Lactobacillus delbrueckii ssp. bulgaricus . However, in spite of its massive use, the physiological state of S. thermophilus in milk has hardly been investigated. We established the first map of the cytosolic proteome of S. thermophilus LMG18311 grown in milk. It comprises 203 identified proteins corresponding to 32% of theoretical proteome. In addition, using proteomic and transcriptomic approaches, we analyzed the physiology of LMG18311 during its late stage of growth in milk (between 2h30 and 5h30). It revealed the up‐regulation of (i) peptides and AA transporters and of specific AA biosynthetic pathways notably for sulfur AA and (ii) genes and proteins involved in the metabolism of various sugars. These two effects were also observed in LMG18311 grown in milk in coculture with L. bulgaricus although the effect on sugar metabolism was less pronounced. It suggests that the stimulatory effect of Lactobacillus on the Streptococcus growth is more complex than AA or peptides supply.

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