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Proteomic analysis of circulating monocytes in Chinese premenopausal females with extremely discordant bone mineral density
Author(s) -
Deng FeiYan,
Liu YaoZhong,
Li LiMing,
Jiang Chen,
Wu Shan,
Chen Yuan,
Jiang Hui,
Yang Fang,
Xiong JiXian,
Xiao Peng,
Xiao SuMei,
Tan LiJun,
Sun Xiao,
Zhu XueZhen,
Liu ManYuan,
Lei ShuFeng,
Chen XiangDing,
Xie JingYun,
Xiao Gary G.,
Liang SongPing,
Deng HongWen
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700480
Subject(s) - bone mineral , pathogenesis , gelsolin , osteoclast , sod2 , osteoporosis , bone remodeling , blot , medicine , gpx1 , endocrinology , chemistry , immunology , biology , glutathione peroxidase , superoxide dismutase , microbiology and biotechnology , oxidative stress , biochemistry , receptor , gene , actin
Osteoporosis (OP) is a major public health problem, mainly characterized by low bone mineral density (BMD). Circulating monocytes (CMCs) may serve as progenitors of osteoclasts and produce a wide variety of factors important to bone metabolism. However, the specific action mechanism of CMCs in the pathogenesis of OP is far from clear. We performed a comparative protein expression profiling study of CMCs in Chinese premenopausal females with extremely discordant BMD, identified a total of 38 differentially expressed proteins, and confirmed with Western blotting five proteins: ras suppressor protein1 (RSU1), gelsolin (GSN), manganese‐containing superoxide dismutase (SOD2), glutathione peroxidase 1(GPX1), and prolyl 4‐hydroxylase β subunit (P4HB). These proteins might affect CMCs' trans ‐endothelium, differentiation, and/or downstream osteoclast functions, thus contribute to differential osteoclastogenesis and finally lead to BMD variation. The findings promote our understanding of the role of CMCs in BMD determination, and provide an insight into the pathogenesis of human OP.