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Early manganese‐toxicity response in Vigna unguiculata L. – a proteomic and transcriptomic study
Author(s) -
Führs Hendrik,
Hartwig Moritz,
Molina Laura Elisa Buitrago,
Heintz Dimitri,
Van Dorsselaer Alain,
Braun HansPeter,
Horst Walter J.
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700478
Subject(s) - vigna , apoplast , photosynthesis , proteome , toxicity , biology , manganese , transcriptome , botany , biochemistry , chemistry , gene expression , gene , cell wall , organic chemistry
The apoplast is known to play a predominant role in the expression of manganese (Mn) toxicity in cowpea ( Vigna unguiculata L.) leaves. To unravel early Mn‐toxicity responses after 1–3 days Mn treatment also in the leaf symplast, we studied the symplastic reactions induced by Mn in two cultivars differing in Mn tolerance on a total cellular level. Comparative proteome analyses of plants exposed to low or high Mn allowed to identify proteins specifically affected by Mn, particularly in the Mn‐sensitive cowpea cultivar. These proteins are involved in CO 2 fixation, stabilization of the Mn cluster of the photosystem II, pathogenesis‐response reactions and protein degradation. Chloroplastic proteins important for CO 2 fixation and photosynthesis were of lower abundance upon Mn stress suggesting scavenging of metabolic energy for a specific stress response. Transcriptome analyses supported these findings, but additionally revealed an upregulation of genes involved in signal transduction only in the Mn‐sensitive cultivar. In conclusion, a coordinated interplay of apoplastic and symplastic reactions seems to be important during the Mn‐stress response in cowpea.