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Quantitative proteomes and in vivo secretomes of progressive and regressive UV‐induced fibrosarcoma tumor cells: Mimicking tumor microenvironment using a dermis‐based cell‐trapped system linked to tissue chamber
Author(s) -
Shi Yang,
Elmets Craig A.,
Smith Jeffery W.,
Liu YuTsueng,
Chen YunRu,
Huang ChengPo,
Zhu Wenhong,
Ananthaswamy Honnavara N.,
Gallo Richard L.,
Huang ChunMing
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700425
Subject(s) - in vivo , proteome , dermis , tumor microenvironment , fibrosarcoma , heat shock protein , biology , proteomics , cell culture , chemistry , microbiology and biotechnology , pathology , biochemistry , immunology , immune system , anatomy , gene , genetics , medicine
Abstract The alterations of tumor proteome and/or in vivo secretome created by host‐tumor cell interaction may be crucial factors for tumors to undergo progression or regression in a host system. Two UV‐induced fibrosarcoma tumor cell lines (UV‐2237 progressive cells and UV‐2240 regressive cells) were used as models to address this issue. Hundreds of proteins including in vivo secretome have been identified and quantified via an isotope‐coded protein label (ICPL) in conjunction with high‐throughput NanoLC‐LTQ MS analysis. A newly designed technology using a dermis‐based cell‐trapped system was employed to encapsulate and grow 3‐D tumor cells. A tissue chamber inserted with a tumor cell‐trapped dermis was implanted into mice to mimic the tumor microenvironment. The in vivo secretome created by host‐tumor interaction was characterized from samples collected from tissue chamber fluids via ICPL labeling mass spectrometric analysis. Twenty‐five proteins including 14‐3‐3 proteins, heat shock proteins, profilin‐1, and a fragment of complement C3 with differential expression in proteomes of UV‐2237 and UV‐2240 cells were revealed. Three secreted proteins including myeloperoxidase, alpha‐2‐macroglobulin, and a vitamin D‐binding protein have different abundances in the in vivo secretome in response to UV‐2237 and UV‐2240 cells. Differential tumor proteomes and in vivo secretome were thus accentuated as potential therapeutic targets to control tumor growth.