Premium
Membrane subproteomic analysis of Mycobacterium bovis bacillus Calmette‐Guérin
Author(s) -
Zheng Jianhua,
Wei Candong,
Leng Wenchuan,
Dong Jie,
Li Rui,
Li Weijun,
Wang Jin,
Zhang Zongde,
Jin Qi
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700342
Subject(s) - mycobacterium bovis , proteome , biology , mycobacterium tuberculosis , microbiology and biotechnology , mycobacterium , membrane protein , bacillus (shape) , tuberculosis , membrane , biochemistry , bacteria , medicine , genetics , pathology
Abstract Mycobacterium bovis bacillus Calmette–Guérin (BCG) vaccine has been known for a long time to prevent tuberculosis (TB) worldwide since 1921. Nonetheless, we know little about BCG membrane proteome. In the present study, we utilized alkaline incubation and Triton X‐114‐based methods to enrich BCG membrane proteins and subsequently digested them using proteolytic enzyme. The recovered peptides were further separated by 2‐D LC and identified by ESI‐MS/MS. As a result, total 474 proteins were identified, including 78 integral membrane proteins (IMPs). Notably, 18 BCG IMPs were described for the first time in mycobacterium. Further analysis of the 78 IMPs indicated that the theoretical molecular mass distribution of them ranged from 8.06 to 167.86 kDa and p I scores ranged from 4.40 to 11.60. Functional classification revealed that a large proportion of the identified IMPs (67.9%, 53 out of 78) were involved in cell wall and cell processes functional group. In conclusion, here we reported a comprehensive profile of the BCG membrane subproteome. The present investigation may allow the identification of some valuable vaccine and drug target candidates and thus provide basement for future designing of preventive, diagnostic, and therapeutic strategies against TB.