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Proteomic analysis of the cellular responses induced in uninfected immune cells by cell‐expressed X4 HIV‐1 envelope
Author(s) -
Molina Laurence,
Grimaldi Marina,
RobertHebmann Véronique,
Espert Lucile,
Varbanov Mihayl,
Devaux Christian,
Granier Claude,
BiardPiechaczyk Martine
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700306
Subject(s) - microbiology and biotechnology , biology , immune system , apoptosis , t cell , programmed cell death , peripheral blood mononuclear cell , cell , cytoskeleton , cell culture , immunology , in vitro , biochemistry , genetics
HIV‐1 envelope gp120 and gp41 glycoproteins (Env), expressed at the cell surface, induce uninfected CD4 T‐cell death, but the molecular mechanisms leading to this demise are still largely unknown. To better understand these events, we analyzed by a proteomic approach the differential protein expression profile of two types of uninfected immune cells after their coculture for 1–3 days with cells that express, or not, Env. First, umbilical cord blood mononuclear cells (UCBMCs) were used to approach the in vivo situation, i.e. , blood uninfected naive cells that encounter infected cells. Second, we used the A2.01/CD4.403 T‐cell line expressing wild type CXCR4 and a truncated form of CD4 that still undergoes Env‐mediated apoptosis, independently of CD4 signaling. After coculture with cells expressing Env, 35 and 39 proteins presenting an altered expression in UCBMCs and the A2.01/CD4.403 T‐cell line, respectively, were identified by mass‐spectrometry. Whatever the cell type analyzed, the majority of these proteins are involved in degradation processes, redox homeostasis, metabolism and cytoskeleton dynamics, and linked to mitochondrial functions. This study provides new insights into the events that sequentially occur in bystander T lymphocytes after contact with HIV‐infected cells and leading, finally, to apoptotic cell death.