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Cytoplasmic proteome reference map for a glutamic acid‐producing Corynebacterium glutamicum ATCC 14067
Author(s) -
Li Liyuan,
Wada Masaru,
Yokota Atsushi
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700269
Subject(s) - corynebacterium glutamicum , proteome , strain (injury) , corynebacterium , glutamic acid , proteomics , biochemistry , biology , chemistry , bacteria , microbiology and biotechnology , amino acid , gene , genetics , anatomy
We constructed a cytoplasmic proteome reference map for a glutamic acid producing Corynebacterium glutamicum ATCC 14067 by 2‐DE and protein identification by MALDI‐TOF‐MS and PMF using genome database of the type strain ATCC 13032. The map allowed us to identify 166 protein spots representing 139 different proteins. A considerable strain difference was observed in the proteomic images between strains ATCC 14067 and ATCC 13032 grown under the glutamic acid production conditions, suggesting the importance of strain‐specific reference map for proteomic analysis.