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Proteomic analysis of the pathophysiological process involved in the antisnake venom effect of Mucuna pruriens extract
Author(s) -
Guerranti Roberto,
Ogueli Ifeanyi G.,
Bertocci Erica,
Muzzi Chiara,
Aguiyi John C.,
Cianti Riccardo,
Armini Alessandro,
Bini Luca,
Leoncini Roberto,
Marinello Enrico,
Pagani Roberto
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700265
Subject(s) - haptoglobin , albumin , venom , pharmacology , in vivo , fibrinogen , mucuna pruriens , blood proteins , inflammation , hemopexin , chemistry , immunology , medicine , biology , biochemistry , traditional medicine , heme , microbiology and biotechnology , enzyme
Previously, we reported the antisnake venom properties of a Mucuna pruriens seed extract (MPE) and tested its in vivo efficacy against Echis carinatus venom (EV) in short‐ (1 injection) and long‐term (three weekly injections) treatments. The aim of the present study was to investigate plasma proteome changes associated with MPE treatments and identify proteins responsible for survival of envenomated mice (CHALLENGED mice). Six treatment groups were studied. Three control groups: one saline, one short‐term and one long‐term MPE treatment. One group received EV alone. Two test groups received EV with either a short‐term or long‐term MPE treatment (CHALLENGED mice). The plasma from each group was analysed by 2‐DE/MALDI‐TOF MS. The most significant changes with treatment were: albumin, haptoglobin, fibrinogen, serum amyloid A and serum amyloid P. Most of these changes were explained by EV effects on coagulation, inflammation and haemolysis. However, MPE treatments prevented the EV‐induced elevation in HPT. Consequently, HPT levels were similar to controls in the plasma of CHALLENGED mice. The plasma of CHALLENGED mice showed substantial proteomic modifications. This suggests the mechanism of MPE protection involves the activation of counterbalancing processes to compensate for the imbalances caused by EV.