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Proteome analysis of aflatoxin B 1 ‐induced hepatocarcinogenesis in tree shrew ( Tupaia belangeri chinensis ) and functional identification of candidate protein peroxiredoxin II
Author(s) -
Li Yuan,
Qin Xue,
Cui Jiefeng,
Dai Zhi,
Kang Xiaonan,
Yue Haiying,
Zhang Yu,
Su Jianjia,
Cao Ji,
Ou Chao,
Yang Chun,
Duan Xiaoxian,
Yue Huifen,
Liu Yinkun
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700229
Subject(s) - peroxiredoxin , western blot , biology , microbiology and biotechnology , hepatocellular carcinoma , apoptosis , cell growth , liver cancer , cancer research , biochemistry , gene , peroxidase , enzyme
In order to explore the proteins responsible for hepatocellular carcinoma (HCC), aflatoxin B 1 ‐induced hepatocarcinogenesis in tree shrew ( Tupaia belangeri chinensis ) was analyzed with 2‐DE and MS. By comparing HCC samples with their own precancerous biopsies and HCC‐surrounding tissues, a group of candidate proteins that differentially expressed in HCC were obtained. Peroxiredoxin (Prx) II, one of the candidates with distinct alteration, was further investigated and validated. Western blot and RT‐PCR assays confirmed the overexpression of Prx II in both tree shrew and human HCC tissues. RNA interference for silencing Prx II was employed subsequently to explore the function and underlying mechanism of Prx II on liver cancer cell line Hep3B. Results showed the cell proliferation and clone formation decreased obviously when Prx II expression was inhibited, while the flow cytometer analysis showed the percentage of cell apoptosis enhanced. Inhibition of Prx II expression also obviously increased the generation of ROS and malondialdehyde, both are the products from peroxidation. These results imply the important role of Prx II in hepatocarcinogenesis, possibly through its function in regulating peroxidation and hereby to provide a favorable microenvironment for cancer cell surviving and progressing.