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Novel identification of expressed genes and functional classification of hypothetical proteins from Neisseria meningitidis serogroup A
Author(s) -
Bernardini Giulia,
Arena Simona,
Braconi Daniela,
Scaloni Andrea,
Santucci Annalisa
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700210
Subject(s) - orfs , proteome , neisseria meningitidis , biology , gene , ribosomal protein , computational biology , genome , proteomics , genetics , identification (biology) , neisseria , peptide sequence , ribosome , open reading frame , bacteria , rna , botany
To implement the 2‐DE database of serogroup A Neisseria meningitidis (MenA) and improve its potential of investigation in bacterial biology, cell extracts were separated by tricine‐SDS‐PAGE and 131 novel proteins were identified by μLC‐ESI‐IT‐MS/MS. These identifications extended to 404, the number of MenA gene expression products characterized at the proteome level, approximately covering 20% of the total ORFs predicted from genome sequence. This technical approach was particularly useful in ascertaining expression of ribosomal as well as hypothetical proteins. Particular attention was paid to functional characterization of hypothetical proteins by means of software analyses and database searches.