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Analysis of differently expressed proteins and transcripts in gills of Penaeus vannamei after yellow head virus infection
Author(s) -
Rattanarojpong Triwit,
Wang HaoChing,
Lo ChuFang,
Flegel Timothy W.
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700202
Subject(s) - arginine kinase , biology , arginine , microbiology and biotechnology , gill , messenger rna , glycolysis , biochemistry , citric acid cycle , virus , enzyme , amino acid , gene , virology , fishery , fish <actinopterygii>
In this proteomic analysis of gills from yellow head virus (YHV)‐infected Penaeus vannamei , we identified 13 spots with up‐regulated protein expression levels and five spots with down‐regulated levels. LC‐nanoESI‐MS/MS indicated that the up‐regulated proteins included enzymes in the glycolytic pathway, the tricarboxylic acid cycle and amino acid metabolism. The other up‐regulated proteins were arginine kinase, imaginal disk growth factor (IDGF) and a Ras‐like GTP binding protein. By contrast, expression levels were reduced for an SCP‐calcium binding protein (SCP), actin‐1, a valosin‐containing protein, and Rab11. Time‐course assays by real time RT‐PCR revealed no significant increase in mRNA level of glycolytic enzymes and arginine kinase. However, a significant decrease in SCP mRNA was observed. The present results are consistent with previously published work and suggest that a decrease in SCP expression may play an important role in the shrimp response to viral infections in general.