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Antibody screening database for protein kinetic modeling
Author(s) -
Spurrier Brett,
Washburn Frank L.,
Asin Samuel,
Ramalingam Sundhar,
Nishizuka Satoshi
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700117
Subject(s) - antibody , computational biology , database , chemistry , computer science , biology , immunology
Knowledge‐based proteomic studies rely on the availability of quality antibodies. The increasing number of commercially available antibodies covers a wide range of protein networks; however, performance of each antibody can vary, depending on what type of cells, treatments, and time points are studied. Here, we describe an antibody database in which we screened 279 antibodies against multiple cell lysates after various treatments and from different time points. We applied these quality‐confirmed antibodies on protein arrays, showing their utility for protein kinetic modeling.