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A la carte proteomics with an emphasis on gel‐free techniques
Author(s) -
Gevaert Kris,
Van Damme Petra,
Ghesquière Bart,
Impens Francis,
Martens Lennart,
Helsens Kenny,
Vandekerckhove Joël
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700114
Subject(s) - proteome , proteomics , notice , posttranslational modification , computational biology , computer science , biology , bioinformatics , biochemistry , political science , law , gene , enzyme
Since the introduction of the proteome term somewhat more than a decade ago the field of proteomics witnessed a rapid growth mainly fueled by instrumental analytical improvements. Of particular notice is the advent of a diverse set of gel‐free proteomics techniques. In this review, we discuss several of these gel‐free techniques both for monitoring protein concentration changes and protein modifications, in particular protein phosphorylation, glycosylation, and protein processing. Furthermore, different approaches for (multiplexed) gel‐free proteome analysis are discussed.