Premium
Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose
Author(s) -
Kim PanKyeom,
Kim MiRyung,
Kim HyunJung,
Yoo HyunSyuk,
Kim JiSoo,
Cho EunHee,
Kim ChanWha
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700051
Subject(s) - hepatic stellate cell , proteome , blot , glycolysis , proteomics , biology , hepatic function , protein expression , biochemistry , carbohydrate metabolism , microbiology and biotechnology , function (biology) , diabetes mellitus , chemistry , medicine , endocrinology , metabolism , gene
Abstract To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2‐DE and ESI‐Q‐TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.