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A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes
Author(s) -
Gloeckner Christian Johannes,
Boldt Karsten,
Schumacher Annette,
Roepman Ronald,
Ueffing Marius
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200700038
Subject(s) - tandem affinity purification , tandem , affinity chromatography , avidity , chemistry , flag tag , protein purification , hek 293 cells , chromatography , computational biology , biochemistry , biology , recombinant dna , enzyme , genetics , gene , antigen , materials science , fusion protein , composite material
Isolation and dissection of native multiprotein complexes is a central theme in functional genomics. The development of the tandem affinity purification (TAP) tag has enabled an efficient and large‐scale purification of native protein complexes. However, the TAP tag features a size of 21 kDa and requires time consuming cleavage. By combining a tandem Strep‐tag II with a FLAG‐tag we were able to reduce the size of the TAP (SF‐TAP) tag to 4.6 kDa. Both moieties have a medium affinity and avidity to their immobilised binding partners. This allows the elution of SF‐tagged proteins under native conditions using desthiobiotin in the first step and the FLAG octapeptide in the second step. The SF‐TAP protocol represents an efficient, fast and straightforward purification of protein complexes from mammalian cells within 2.5 h. The power of this novel method is demonstrated by the purification of Raf associated protein complexes from HEK293 cells and subsequent analysis of their protein interaction network by dissection of interaction patterns from the Raf binding partners MEK1 and 14‐3‐3.