Premium
Sensitive, Quantitative, and Fast Modifications for Coomassie Blue Staining of Polyacrylamide Gels
Author(s) -
Westermeier Reiner
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200690121
Subject(s) - staining , coomassie brilliant blue , silver stain , polyacrylamide , chromatography , polyacrylamide gel electrophoresis , mass spectrometry , chemistry , two dimensional gel electrophoresis , gel electrophoresis , proteomics , microbiology and biotechnology , biochemistry , biology , genetics , gene , polymer chemistry , enzyme
In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There are several reasons:Low price Visible with the eye Desk top scanners can be employed for image acquisition Better for quantitative analysis than silver staining Possible modifications for fast or highly sensitive staining Mass spectrometry compatible