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Preparation of Plant Samples for 2‐D Electrophoresis
Author(s) -
Westermeier Reiner
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200690120
Subject(s) - electrophoresis , chromatography , gel electrophoresis , polyphenol , chemistry , two dimensional gel electrophoresis , polysaccharide , gel electrophoresis of proteins , proteomics , polyacrylamide gel electrophoresis , biochemistry , enzyme , gene , antioxidant
Plant protein samples are very difficult to extract and prepare for 2‐D electrophoresis, because polyphenols can build irreversible complexes with the proteins, the protein concentrations are relatively low, polysaccharides and lipids can cause severe disturbances in the 2‐D gel.