Premium
Broad spectrum identification of SUMO substrates in melanoma cells
Author(s) -
Ganesan Anand K.,
Kho Yoonjung,
Kim Sung Chan,
Chen Yue,
Zhao Yingming,
White Michael A.
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600971
Subject(s) - sumo protein , microbiology and biotechnology , phosphorylation , biology , signal transduction , proteomics , broad spectrum , epidermal growth factor , function (biology) , cell growth , ubiquitin , chemistry , cell culture , genetics , gene , combinatorial chemistry
Like phosphorylation, protein sumoylation likely represents a dynamic PTM to alter protein function in support of cell regulatory systems. The broad‐spectrum impact of transient or chronic engagement of signal transduction cascades on protein sumoylation has not been explored. Here, we find that epidermal growth factor (EGF) stimulation evokes a rapid alteration in small ubiquitin modifier (SUMO) target selection, while oncogene expression alters steady‐state SUMO‐protein profiles. A proteomic SUMO target analysis in melanoma cells identified proteins involved in cellular signaling, growth control, and neural differentiation.