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Proteomic analysis of roots growth and metabolic changes under phosphorus deficit in maize ( Zea mays L.) plants
Author(s) -
Li Kunpeng,
Xu Changzheng,
Zhang Kewei,
Yang Aifang,
Zhang Juren
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600960
Subject(s) - proteome , biology , metabolism , protein biosynthesis , zea mays , signal transduction , metabolic pathway , biochemistry , function (biology) , pi , plant physiology , biosynthesis , gene , gene expression , proteomics , microbiology and biotechnology , botany , agronomy
Phosphorus (P) deficiency is a major limitation for plant growth and development. Plants can respond defensively to this stress, modifying their metabolic pathways and root morphology, and this involves changes in their gene expression. To better understand the low P adaptive mechanism of crops, we conducted the comparative proteome analysis for proteins isolated from maize roots treated with 1000 μM (control) or 5 μM KH 2 PO 4 for 17 days. The results showed that approximately 20% of detected proteins on 2‐DE gels were increased or decreased by two‐fold or more under phosphate (Pi) stress. We identified 106 differentially expressed proteins by MALDI‐TOF MS. Analysis of these P starvation responsive proteins suggested that they were involved in phytohormone biosynthesis, carbon and energy metabolisms, protein synthesis and fate, signal transduction, cell cycle, cellular organization, defense, secondary metabolism, etc. It could be concluded that they may play important roles in sensing the change of external Pi concentration and regulating complex adaptation activities for Pi deprivation to facilitate P homeostasis. Simultaneously, as a basic platform, the results would also be useful for the further characterization of gene function in plant P nutrition.