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Suction blister fluid as potential body fluid for biomarker proteins
Author(s) -
Kool Jeroen,
Reubsaet Léon,
Wesseldijk Feikje,
Maravilha Raquel T.,
Pinkse Martijn W.,
D'Santos Clive S.,
van Hilten Jacobus J.,
Zijlstra Freek J.,
Heck Albert J. R.
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600938
Subject(s) - proteome , suction blister , biomarker , suction , biological fluids , body fluid , biomarker discovery , interstitial fluid , proteomics , computational biology , medicine , biology , bioinformatics , chemistry , pathology , chromatography , surgery , biochemistry , mechanical engineering , gene , engineering
Early diagnosis is important for effective disease management. Measurement of biomarkers present at the local level of the skin could be advantageous in facilitating the diagnostic process. The analysis of the proteome of suction blister fluid, representative for the interstitial fluid of the skin, is therefore a desirable first step in the search for potential biomarkers involved in biological pathways of particular diseases. Here, we describe a global analysis of the suction blister fluid proteome as potential body fluid for biomarker proteins. The suction blister fluid proteome was compared with a serum proteome analyzed using identical protocols. By using stringent criteria allowing less than 1% false positive identifications, we were able to detect, using identical experimental conditions and amount of starting material, 401 proteins in suction blister fluid and 240 proteins in serum. As a major result of our analysis we construct a prejudiced list of 34 proteins, relatively highly and uniquely detected in suction blister fluid as compared to serum, with established and putative characteristics as biomarkers. We conclude that suction blister fluid might potentially serve as a good alternative biomarker body fluid for diseases that involve the skin.