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Proteome analysis of Streptococcus suis serotype 2
Author(s) -
Jing HongBo,
Yuan Jing,
Wang Jie,
Yuan Yuan,
Zhu Li,
Liu XianKai,
Zheng YuLing,
Wei KaiHua,
Zhang XueMin,
Geng HongRan,
Duan Qing,
Feng ShuZhang,
Yang RuiFu,
Cao WuChun,
Wang HengLiang,
Jiang YongQiang
Publication year - 2008
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600930
Subject(s) - streptococcus suis , arginine deiminase , biology , virulence , proteome , virulence factor , microbiology and biotechnology , proteomics , streptococcus pyogenes , biochemistry , enolase , arginine , bacteria , genetics , amino acid , gene , staphylococcus aureus , immunohistochemistry , immunology
Outbreaks in humans, caused by Streptococcus suis serotype 2 (SS2), were reported in 1998 and 2005 in China. However, the mechanism of SS2‐associated infection remains unclear. For the first time, a 2‐D gel approach combined with MS was used to establish a comprehensive 2‐D reference map for aiding our understanding of the pathogenicity of SS2. The identification of 694 out of 834 processed spots revealed 373 proteins. Most of the identified proteins were located in the cytoplasm and were involved in energy metabolism, protein synthesis, and cellular processes. Proteins that were abundant in the 2‐DE gels could be linked mainly to housekeeping functions in carbohydrate metabolism, protein quality control and translation. 2‐DE of secretory proteins was performed using IPG strips of pH 4–7. Among the 102 protein spots processed, 87 spots representing 77 proteins were successfully identified. Some virulence‐associated proteins of SS2 were found, including arginine deiminase, ornithine carbamoyl‐transferase, carbamate kinase, muramidase‐released protein precursor, extracellur factor, and suilysin. Enolase and endopeptidase have been proposed as putative virulence‐associated factors in this study. The 2‐D reference map might provide a powerful tool for analyzing the virulence factor and the regulatory network involved in the pathogenicity of this microorganism.