Premium
A proteomic approach to studying the differentiation of neural stem cells
Author(s) -
Skalnikova Helena,
Halada Petr,
Vodicka Petr,
Motlik Jan,
Rehulka Pavel,
Hørning Ole,
Chmelik Josef,
Nørregaard Jensen Ole,
Kovarova Hana
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600867
Subject(s) - biology , neural stem cell , neurogenesis , microbiology and biotechnology , cellular differentiation , proteomics , heterogeneous nuclear ribonucleoprotein , neural development , neurosphere , rna binding protein , rna , stem cell , ribonucleoprotein , biochemistry , adult stem cell , gene
Abstract The mechanisms that regulate the maintenance of stem cell self‐renewal versus differentiation are complex and remain mostly unknown. Understanding neurogenesis and neural cell differentiation presents a unique challenge for the treatment of nervous system disorders. To gain more insight into molecular mechanisms of the differentiation of neural cells, we combined the advantage of porcine fetal neural stem cells (NSCs) in vitro differentiation model and proteomic analysis. Using 2‐DE followed by MS, we profiled constituent proteins of NSCs and their differentiated progenies at first and then indicated protein species that were significantly up‐ or down‐regulated during the differentiation. The largest identified group of constituent proteins was related to RNA and protein metabolism and processing, including chaperones, and the second largest consisted of proteins involved in cell organization (cytoskeleton and annexins). Differentiation of neural cells was found to be accompanied by changes in the expression of proteins involved in DNA and RNA binding, mRNA processing and transport, stress responses, iron storage, and redox regulation. Additional immunoblot analysis verified the induction of α‐B crystallin and heterogenous nuclear ribonucleoproteins (hnRNPs) A1 and A2/B1. Furthermore, immunocytochemistry demonstrated specific localization of α‐B crystallin in the cytoplasm or nucleus of glial cells and confirmed cellular expression patterns of hnRNPs A1 and A2/B1. These findings represent a significant step towards understanding neural cell differentiation and identification of the regulatory proteins associated with this process.