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A two‐dimensional proteome reference map of Herbaspirillum seropedicae proteins
Author(s) -
Chaves Daniela Fojo Seixas,
Ferrer Pércio Pereira,
de Souza Emanuel Maltempi,
Gruz Leonardo Magalhães,
Monteiro Rose Adele,
de Oliveira Pedrosa Fábio
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600859
Subject(s) - orfs , diazotroph , proteome , biology , genome , computational biology , gene , genetics , proteomics , open reading frame , bacteria , peptide sequence , nitrogen fixation
Herbaspirillum seropedicae is an endophytic diazotroph associated with economically important crops such as rice, sugarcane, and wheat. Here, we present a 2‐D reference map for H. seropedicae . Using MALDI‐TOF‐MS we identified 205 spots representing 173 different proteins with a calculated average of 1.18 proteins/gene. Seventeen hypothetical or conserved hypothetical ORFs were shown to code for true gene products. These data will support the genome annotation process and provide a basis on which to undertake comparative proteomic studies.