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A comprehensive proteome map of the lipid‐requiring nosocomial pathogen Corynebacterium jeikeium K411
Author(s) -
Hansmeier Nicole,
Chao TzuChiao,
Daschkey Svenja,
Müsken Mathias,
Kalinowski Jörn,
Pühler Alfred,
Tauch Andreas
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600833
Subject(s) - proteome , biochemistry , biology , microbiology and biotechnology , virulence , proteomics , pathogen , gene
Corynebacterium jeikeium is a lipid‐requiring pathogen that is considered as part of the normal microflora of the human skin and associated with severe nosocomial infections. Systematic reference maps of the cytoplasmic, cell surface‐associated, and extracellular proteome fractions of the clinical isolate C. jeikeium K411 were examined by 2‐DE coupled with MALDI‐TOF MS. A sum total of 555 protein spots were identified by PMF, corresponding to 358 different proteins that were classified into functional categories and integrated into metabolic pathways. The majority of the proteins were linked to housekeeping functions in energy production and translation and to physiological processes in amino acid, carbohydrate, nucleotide, and lipid metabolism. A complete enzymatic machinery necessary to utilize exogenous fatty acids by β‐oxidation was detected in the cytoplasmic proteome fraction. In addition, several predicted virulence factors of C. jeikeium K411 were identified in the cell surface‐associated and extracellular subproteome, including the cell surface proteins SurA and SurB, the surface‐anchored pilus subunits SapA and SapB, the surface‐anchored collagen adhesin CbpA, the cholesterol esterase Che, and the acid phosphatase AcpA.