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Proteome analysis of whole saliva: A new tool for rheumatic diseases – the example of Sjögren's syndrome
Author(s) -
Giusti Laura,
Baldini Chiara,
Bazzichi Laura,
Ciregia Federica,
Tonazzini Ilaria,
Mascia Giovanni,
Giannaccini Gino,
Bombardieri Stefano,
Lucacchini Antonio
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600783
Subject(s) - saliva , proteome , proteomics , connective tissue , inflammation , medicine , immunology , oxidative stress , biology , pathology , bioinformatics , biochemistry , gene
Abstract Sjögren's syndrome (pSS) is a systemic disease that affects salivary glands directly, and is therefore expected to influence the composition of human whole saliva (WS) fluid. The aim of this study was to characterize the WS proteins of pSS patients using a proteomic approach to assess a valid procedure to examine the global changes of the salivary protein profiles in connective tissue disorders. The WS proteins expressed in patients affected by pSS and healthy volunteers were analyzed using the 2‐DE technique. The WS protein pattern was altered in pSS patients compared to controls, with a decrease in some of the typical salivary proteins. Particularly, a remarkable alteration of carbonic anhydrase VI was observed. Moreover, a comparison of WS protein profile of pSS patients with the one obtained from controls revealed a set of differentially expressed proteins. These proteins were related to acute and chronic inflammation while some others were involved in oxidative stress injury. These findings are in line with the systemic immunoinflammatory aspects of pSS and open the possibility for a systematic search of diagnostic biomarkers and targets for therapeutic intervention in pSS.