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iTRAQ compatibility of peptide immobilized pH gradient isoelectric focusing
Author(s) -
Lengqvist Johan,
Uhlén Kristina,
Lehtiö Janne
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600782
Subject(s) - isoelectric focusing , immobilized ph gradient , chromatography , fractionation , chemistry , proteome , compatibility (geochemistry) , proteomics , isoelectric point , peptide , biochemistry , materials science , composite material , gene , enzyme
Immobilized pH gradient isoelectric focusing (IPG‐IEF) has emerged as a highly promising alternative to strong‐cation exchange fractionation as the first dimension in shot‐gun proteomics. Herein is shown the compatibility of this method with iTRAQ isotope labeling for relative quantitation and validation of sequence matches from database searching.