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Proteomic analysis of Burkholderia cepacia MBA4 in the degradation of monochloroacetate
Author(s) -
Kwok SuiYi,
Siu Annie FungMing,
Ngai SaiMing,
Che ChiMing,
Tsang Jimmy S. H.
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600660
Subject(s) - burkholderia , edman degradation , bacteria , biochemistry , amino acid , microbiology and biotechnology , biology , proteomics , chemistry , peptide sequence , gene , genetics
Burkholderia cepacia MBA4 is a bacterium that degrades 2‐haloacids by removing the halogen and subsequent metabolism of the product for energy. In this study, 2‐DE, MS/MS, and N‐terminal amino acid sequencing were used to investigate the protein expression profiles of MBA4 grown in a 2‐haloacid (monochloroacetate, MCA) and in the corresponding metabolic product (glycolate). Glycolate was used as a control to eliminate the proteins induced by it. Five proteins were found to be up‐regulated and five proteins were down‐regulated in response to MCA. The differentially expressed proteins were examined, seven of them were identified by MS/MS and two of them were sequenced by Edman degradation. Our results definitely provide an insight for understanding the physiology of B. cepacia MBA4 in response to organohalide contaminated site.